Taulmaran Deep sequencing has previously been used to characterize cultured bacterial isolates 27 and to explore the composition of microbial populations in metagenomic research 28 Here, we utilized deep sequencing of 16S rRNA to implicate Actinomadura madurae as the cause of mycetoma in a diabetic patient when culture and conventional molecular methods were overwhelmed by overgrowth of other organisms. Application of 16S rRNA gene sequencing to identify Bordetella hinzii as the causative agent of fatal septicemia. No new lesions evolved while she was on this therapy, but approximately 1 year prior to presentation the patient self-discontinued ciprofloxacin and shortly thereafter noted increasing drainage from her foot. Although the application of deep sequencing to microbial communities traces its origins to metagenomics research 13we recently demonstrated the feasibility of using deep sequencing to interrogate the composition of polymicrobial specimens in a clinical context by sequencing bacterial 16S rRNA amplified directly from patient material 3.

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Support Center Support Center. Deep sequencing successfully detected the presence of A. DNA was amplified from the specimen in two wctinomadura reactions, in which sequencing adapters were incorporated on opposite ends of the amplicon, enabling bidirectional sequencing across the full length of 16S rRNA variable regions 1 and 2 3. Impact of 16S rRNA gene sequence analysis for identification of bacteria on clinical microbiology and infectious diseases.

Treatment of actinomycetoma foot—our experience with ten patients. The portion of the biopsy material sequenced may have contained little of the aerobic actinomycetes inclusion, which represented a focal area within the larger sample Fig. For some sequences, multiple organisms met the identity threshold for species-level classification; in such actinomadua, species names separated by a slash and genus names separated by a semicolon list the possible taxonomic assignments.

Author information Copyright and License information Disclaimer. A punch biopsy specimen of the lesion was submitted to pathology, revealing actinomadurw acute and chronic inflammation and an inclusion of abundant filamentous structures consistent with aerobic actinomycetes Fig.

Journal List J Clin Microbiol v. Despite treatment, the lesions continued to steadily grow, and 2 years prior to presentation she received a 6-week course of intravenous penicillin therapy with continuous daily infusions 20 million units intravenously [i. A second biopsy specimen aactinomadura submitted for culture and was again positive for S. You can help Wikipedia by expanding it. Based on this information, a course of sulfamethoxazole-trimethoprim therapy was considered.

Metagenomic systems biology of the human gut microbiome reveals topological shifts associated with obesity and inflammatory bowel disease. Thus, the relative abundance of A. Several treatment regimens for actinomycotic mycetoma have been published 18 — 20and in general, the condition shows response to a wide range of antibiotics, including aminoglycosides, rifampin, amoxicillin-clavulanic acid, doxycycline, and sulfamethoxazole-trimethoprim, nadurae combination antibiotic therapy is recommended Actinomadura madurae is an aerobic actinomycete which is best known worldwide as the cause of actinomycotic mycetomas.

The patient noted some retreat of the lesions and improvement in her pain and pruritis while on this treatment, and she was transitioned to oral penicillin mg p. It has not previously been reported to have caused invasive pulmonary or disseminated infection in humans.

Consistent with culture results, reads classified as S. Table 1 Deep-sequencing results. From Wikipedia, the free encyclopedia. Given the extent of infection, neither additional antibiotic madurad nor debridement with subsequent reconstruction was felt to be a viable option, and a recommendation was made for below-the-knee amputation. A number of other organisms were also detected, reflecting the polymicrobial nature of such specimens 5.

A Punch biopsy material stained using hematoxylin and eosin. There was a problem providing the content you requested Although the application of deep sequencing to microbial communities traces its origins to metagenomics research 13we recently demonstrated the feasibility of using deep sequencing to interrogate the composition of polymicrobial specimens in a clinical context by sequencing bacterial 16S rRNA amplified directly from patient material 3.

The tallies and percent abundance of reads corresponding to actlnomadura organism are indicated. An inclusion of filamentous, basophilic organisms consistent with Actinomyces species is seen.

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Keratinocyte infection by Actinomadura madurae triggers an inflammatory response.

Die Actinobacteria sind grampositive Bakterien. Hierbei fallen viele Vertreter der Actinobacteria bereits auf, da ihre Kolonien nicht glatt, sondern runzlig aussehen. Dies trifft auf die Mehrzahl der Vertreter zu, allerdings nicht auf alle, so dass diese Definition allein nicht ausreicht. Der Stoffwechsel der meisten Vertreter der Actinobacteria ist als chemoorganotroph und heterotroph zu kennzeichnen, sie benutzen organische Verbindungen als Energiequelle und ebenso zum Aufbau zelleigener Stoffe. So findet sich unter anderem bei den Vertretern der Familie der Mycobacteriaceae Mykobakterien ein hoher Lipidanteil im Peptidoglycan der bakteriellen Zellwand.


Nonmycetomic Actinomadura madurae infection in a patient with AIDS.

Description and Natural Habitats Actinomadura is a filamentous bacterium found in soil. Although it was once believed to be a fungus, the information later attained about its ultrastructural cellular properties showed that Actinomadura is in fact an aerobic actinomycetes. However, for the reason that most of the diagnostic procedures related to Actinomadura are still held in mycology laboratories in many centers, the discussion on Actinomadura is included in this website. Species Actinomadura madurae, Actinomadura pelletieri, and Actinomadura dassonvillei are the species included in the genus Actinomadura. Actinomadura madurae is distinguished from A. Pathogenicity and Clinical Significance It is one of the common causes of actinomycotic mycetoma maduramycosis or madura foot , characterized by formation of granules containing branched filaments.

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